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Biotechnology Engineering · Q53

Recombinant DNA Technology

Engineering and GATE · Biotechnology Engineering · question 53

Q53

"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments. Plasmid + HindIII → 1200bp and 3800bp Plasmid + BamHI → 5000bp Plasmid + EcoRI → 2500bp The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"

A.
EcoRI-2; BamHI-1; HindIII-2
Answer
B.
EcoRI-1; BamHI-1; HindIII-2
C.
EcoRI-3; BamHI-2; HindIII-1
D.
EcoRI-2; BamHI-2; HindIII-1

Answer: Option A

Solution

Answer: Option A
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