Q23
A DNA fragment of 4500 bp has to be tailed with dT residues by using dTTP and the enzyme 'terminal transferase' The stock solution of dTTP that is used as a substrate has concentration of 150 μM. Ten μL of this stock solution is added to a total volume of 200 μL reaction. What will be the concentration of dTTP in the reaction?
A.
7.5 μM
AnswerB.
75 μM
C.
0.75 μM
D.
0.075 μM
Answer: Option A
Solution
Answer: Option A
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