Q2
A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain of E. coli having the genotype lacI+ crp+. Even in the absence of inducer IPTG, low levels of expression of the recombinant protein are seen (leaky expression). Which one of the following should be done to minimize such leaky expression?
A.
Addition of lactose to the medium
B.
Removal of all glucose from the medium
C.
Addition of excess glucose to the medium
AnswerD.
Addition of allo-lactose to the medium
Answer: Option C
Solution
Answer: Option C
No explanation is given for this question Let's Discuss on Board