Vidyalelo
Agriculture · Q829

Plant Pathology

Graduate and Post Graduate · Agriculture · question 829

Q829

Concentration of agar in pour plate technique is

A.
2%
B.
1%
C.
1.5%
Answer
D.
0.5%

Answer: Option C

Solution

Answer: Option C
Solution:
Concentration of agar in the pour plate technique is 1.5%.
Agar is typically used in a final concentration of 1 - 2% for solidifying culture media. Smaller quantities (0.05 - 0.5%) are used in media for motility studies and the growth of anaerobes (0.1%) and microaerophiles.
In these media, the agar which is an unbranched long chain of polysaccharides is added in the concentration of 1.5. Most commonly, 1.3% agar is used to prepare solid media in labs.