Q7
Examine the data given below in the table on purification of a protein X. Step Volume (mL) Protein Concentration (mg mL-1) Enzyme Activity (unit mL-1) Crude cell-free extract 500 12.0 5.0 Ammonium sulfate precipitation 125 3.0 11.0 Ion-exchange chromatography 10 9.0 75.0 The yield percent and purification factor, respectively, at the end of the experiment will be approximately
Examine the data given below in the table on purification of a protein X.
The yield percent and purification factor, respectively, at the end of the experiment will be approximately
| Step | Volume (mL) | Protein Concentration (mg mL-1) | Enzyme Activity (unit mL-1) |
| Crude cell-free extract | 500 | 12.0 | 5.0 |
| Ammonium sulfate precipitation | 125 | 3.0 | 11.0 |
| Ion-exchange chromatography | 10 | 9.0 | 75.0 |
The yield percent and purification factor, respectively, at the end of the experiment will be approximately
A.
25 and 3
B.
30 and 20
AnswerC.
20 and 30
D.
75 and 30
Answer: Option B
Solution
Answer: Option B
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