Systematic hybridization on a scientific basis began with the work

Answer: Option C

Systematic hybridization on a scientific basis began with the work Koelreuter-Nicotiana.
Kolreuter began with the systematic study of plant hybridization.
This author performed crosses between Nicotiana paniculata and N. rustica (Kolreuter in 1761). As a result of these crosses, he made important conclusions about plant hybridization.