Vidyalelo
Agriculture · Q558

Plant Pathology

Graduate and Post Graduate · Agriculture · question 558

Q558

The mordant used in Gram staining is

A.
Safranine
B.
Crystal violet
C.
Iodine
Answer
D.
Ethanol

Answer: Option C

Solution

Answer: Option C
Solution:
The mordant used in Gram staining is Iodine.
The mordant is Gram's Iodine. This binds to the crystal violet making a large complex that adheres to the cell membrane.
Gram's Iodine is allowed to sit for 30 seconds then the decolourizer, 95% ethanol is added.