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Biotechnology Engineering · Q4

Recombinant DNA Technology

Engineering and GATE · Biotechnology Engineering · question 4

Q4

The schematic of a plasmid with a gap in one of the strands is shown below: Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid? P. DNA ligase Q. Alkaline phosphatase R. DNA polymerase S. Polynucleotide kinase

A.
P only
B.
P, R and S only
Answer
C.
P and R only
D.
P, Q and R only

Answer: Option B

Solution

Answer: Option B
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