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Biotechnology Engineering · Q12

Recombinant DNA Technology

Engineering and GATE · Biotechnology Engineering · question 12

Q12

Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because

A.
The sequence context at the RB defines the direction of transfer
Answer
B.
The sequence context at the LB defines the direction of transfer
C.
The nuclear location sequence (NLS) of VirD2 protein drives the excised T-strand
D.
The endonuclease activity of VirD2 protein allows nicking at RB

Answer: Option A

Solution

Answer: Option A
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