Who produced puppies through artificial insemination in dogs

Answer: Option C

Spallanzani produced puppies through artificial insemination in dogs.
In Artificial Insemination (AI) the semen is collected manually from a stud male and thereafter deposited (inseminated) in the female so that fertilization can occur in the absence of natural mating.
This first AI was performed by Lazzaro Spallanzani in 1780 by inseminating a bitch which later gave birth to three pups. He is also called as “Father of modern artificial insemination”.