Q41
Bacillus subtilis under ideal conditions occupies 5% of the total volume of a fermenter. Under such conditions; the culture medium (after bacilli are filtered off) has a concentration of 1mg/ml of the secreted protein trehalae (i) Calculate the volume of culture filtrate required to purify 1 kg of trehalase (ii) What are the total number of B. subtilis cells which produce this amount of enzyme? Assume that each cell is a cylinder which has dimensions of 1 m diameter and length 2μm.
A.
(i) 106 ml, (ii) 1.57 × 108
B.
(i) 108 ml, (ii) 3.16 × 1018
C.
(i) 105 ml, (ii) 1.57 × 108
D.
(i) 106 ml, (ii) 3.18 × 1016
AnswerAnswer: Option D
Solution
Answer: Option D
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