What is the primary purpose of neomycin in creating mice with knockouts in gene X?
A. Neomycin selects for the survival of embryonic stem cells (ES) that have incorporated the mutant gene X anywhere in the genome
B. Neomycin selects for the survival of ES cell that have incorporated the mutant gene in the place of the wild-type gene
C. Neomycin prevents Candida infection during ES cell culture that does not have gene X
D. Neomycin makes the gene X knock-out mice resistant to Candida infection
Select an option to see the answer and solution.
The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilized to
A. Remove large proteins
B. Remove collagen only
C. Remove mycoplasma and microorganisms
D. Remove foaming agents
Select an option to see the answer and solution.
During the media preparation for cultivation of cells, insoluble precipitates of calcium phosphates are often formed, identify which method can be adopted to avoid this problem?
A. Hold the pH at 5.6
B. Hold the pH at 7.5
C. Add calcium salt first and then phosphate source
D. None of the above
Select an option to see the answer and solution.
To create a transgenic organism:
A. Introduce foreign DNA into a gamete or fertilized ovum
B. Inject a gene of interest into a somatic cell
C. Inject a gene of interest into several somatic cells
D. Introduce foreign DNA into somatic cells in culture and transplant them
Select an option to see the answer and solution.
When scientists make an animal superior by view of genotype, introducing some foreign genes in it, the phenomenon is called
A. Tissue culture
B. Biotechnology
C. Genetic engineering
D. Immunisation
Select an option to see the answer and solution.
The following culture systems are used for growing large amount of anchorage dependent animal cells except
A. Roller bottle
B. Airlift fermenter
C. Hollow fiber reactor
D. Microcarriers
Select an option to see the answer and solution.
Which of the following promotes glucose and amino acid uptake by muscle?
A. Adrenaline
B. Insulin
C. Glucagon
D. Cortisol
Select an option to see the answer and solution.
Mitochondrial DNA is replicated from
A. A single ori site bidirectionally
B. Two different ori sites in the same direction
C. Two different ori sites at different times in opposite directions
D. Many sites bidirectionally, like nuclear chromosomes
Select an option to see the answer and solution.
In transgenics, alteration in the sequence of nucleotide in genes are due to
I. Subsitution
II. Deletion
II. Insertion
IV. Rearrangement
A. I and II
B. I, II and III
C. II and III
D. III and IV
Select an option to see the answer and solution.
The sarcoplasmic reticulum in muscle cells acts as a
A. Store of digestive enzymes
B. Store of sodium ions
C. Store of lipid
D. Store of calcium ions
Select an option to see the answer and solution.
A molecule that consists of a piece of DNA from one organism, combined with the DNA from a member of another species is called
A. Restricted DNA
B. Recombinant DNA
C. Transgenic DNA
D. Bioengineered DNA
Select an option to see the answer and solution.
Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell?
A. Electroporation
B. Liposome transfer
C. Microinjection
D. Particle bombardment
Select an option to see the answer and solution.
Identify the correct statements for the 'HAT medium'.
P. Includes drug aminopterin to block major pathway for synthesis of deoxyribonucleotides.
Q. Hypoxanthine is precursor for thymidine.
R. Includes drug aminopterin to block major pathway for synthesis of polypeptides.
S. Cells can grow in presence of aminopterin only if they have enzymes thymidine kinase and hypoxanthine-guanine phosphoribosyl transferase.
Select an option to see the answer and solution.
In general, the higher the intensity of exercise, the greater the proportional contribution of
A. Aerobic energy production
B. Anaerobic energy production
C. The TCA cycle (Krebs' cycle) to the production of ATP
D. The electro n transfer chain to the production of ATP
Select an option to see the answer and solution.
The conversion of one molecule of glucose to two molecules of pyruvate results in the net formation of
A. Six molecules of water
B. Two molecules of ATP
C. Three molecules of ATP
D. Thirty-eight molecules of ATP
Select an option to see the answer and solution.
Which one of the following is used as a pH indicator in animal cell culture medium?
A. Acridine orange
B. Phenol red
C. Bromophenol blue
D. Coomassie blue
Select an option to see the answer and solution.
A neonatally thymectomized mouse immunized with protein antigen shows
A. Both primary and secondary responses to antigen
B. Only primary response to antigen
C. Delayed type hypersensitive reactions
D. No response to the antigen
Select an option to see the answer and solution.
Which of the following releases most energy when completely oxidised in the body?
A. One gram of glucose
B. One gram of palmitic acid
C. One gram of proteins
D. One gram of alcohol
Select an option to see the answer and solution.
Which of the following reagents is used for harvesting anchorage-dependent animal cells from culture vessels?
A. Trypsin/Collagenase
B. Trypsin/Collag
C. Collagen/Fibronectin
D. DMSO
Select an option to see the answer and solution.
Which of the following statements regarding the supercoiling of duplex DNA is correct?
A. DNA replication promotes positive supercoiling downstream from the replication fork
B. DNA polymerase III can replicate positively supercoiled duplex DNA
C. DNA topoisomerases unwind duplex DNA into single-stranded DNA chains
D. DNA topoisomerases unwind positively supercoiled duplex DNA without cutting the phosphosugar backbone
Select an option to see the answer and solution.