Q39
The DNA sequence shown below is to be amplified by PCR: 5' GCTAAGATCTGAATTTTCC . . . . . . . . TTGGGCAATAATGTAGCGC 3' 3' CGATTCTAGACTTAAAAGG . . . . . . . . AACCCGTTATTACATCGCG 5' Which one of the following pair of primers can be used for this amplification?
A.
5' GGAAATTCAGATCTTAGT 3' and 5' TGGGCAATAATGTAGCGC 3'
B.
5' GCTAAGATCTGAATTTTC 3' and 5' GCGCTACATTATTGCCCA 3'
AnswerC.
5' CGGAAATTCAGATCTTAG 3' and 5' GCGCTACATTATTGCCCA 3'
D.
5' GCTAAGATCTGAATTTTC 3' and 5' TGGGCAATAATGTAGCGC 3'
Answer: Option B
Solution
Answer: Option B
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