Expression of hundreds of different genes in DNA microarray technology is monitored by using
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Biotechnology Engineering · all questionsMolecular Tools in Biotechnology
Molecular Tools in Biotechnology
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Identical sized RNA transcript is detected by Northern blot analysis of UDP glucuronosyl transferase obtained from human liver and kidney. Microarray analysis of the same samples shows equal spot intensity, whereas Western blot detects a 55 kDa strong band in liver, but a very faint in kidney of same size. The regulation of UDP glucuronosyl transferase is
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Which one of the following techniques/tools is not used for inserting a foreign gene into a cell?
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Which one of the following techniques can be used to compare the expression of a large number of genes in two biological samples in a single experiment?
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The nucleotide analogue used in DNA sequencing by chain termination method is
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Expression of which of the following reporter genes do not require addition of specific substrate for detection?
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What will be the binding status of regulatory proteins in lac operon when concentrations of both lactose and glucose are very low in the culture medium?
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Match the items in Group I with correct options in Group II.
| Group I | Group II |
| P. DNA footprinting | 1. Protein-protein interaction |
| Q. Yeast two-hybrid system | 2. VNTR |
| R. DNA fingerprinting | 3. DNA binding protein |
| S. SAGE | 4. Transcriptome analysis |
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A thermostable DNA polymerase that can carry out both reverse transcription reaction and polymerization has been isolated form
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A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg2+. Which one of the following is missing in the reaction mixture?
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Enzyme used in 'cycle' sequencing of DNA is
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Which one of the following is not a requirement for a PCR reaction?
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Which of the following statements about gene therapy are correct?
P. Affected individuals, but not their progeny, can be cured through germline gene therapy.
Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy.
R. Affected individuals, but not their progeny, can be cured through somatic gene therapy.
S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.
P. Affected individuals, but not their progeny, can be cured through germline gene therapy.
Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy.
R. Affected individuals, but not their progeny, can be cured through somatic gene therapy.
S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.
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Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction?
P. Mg2+ concentration
Q. pH
R. Annealing temperature
P. Mg2+ concentration
Q. pH
R. Annealing temperature
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The heterozygosity of any locus can be ascertained by
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For PCR to amplify the sequence
ATCTTCTACG . . . . . . . . AAGCPTGCGG
TAGAAGATGC . . . . . . . .TTCGAACGCC
The required primers are
ATCTTCTACG . . . . . . . . AAGCPTGCGG
TAGAAGATGC . . . . . . . .TTCGAACGCC
The required primers are
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In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be
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In human populations 4% of the individuals are homozygous recessive to a specific RFLP marker. What percentage of individuals does you expect to be heterozygous for this trait?
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Which of the following fluorescent probes is used to monitor the progress of amplification in Real time PCR?
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Match items in Group I with correct options from those given in Group II.
| Group I | Group II |
| P. VNTR sequence | 1. Gene regulation on the same chromosome |
| Q. Leader sequence | 2. Ribosome binding site |
| R. SD sequence | 3. DNA fingerprinting |
| S. cis-acting sequence | 4. Functions in attenuation |
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