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Biotechnology Engineering · Q29

Methods and Techniques in Applied Biology

Engineering and GATE · Biotechnology Engineering · question 29

Q29

The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?

A.
EcoRI (G/AATTC)
B.
XmaIII (C/GGCGG)
Answer
C.
SmaI (CCC/GGG)
D.
SacII (CCGC/GG)

Answer: Option B

Solution

Answer: Option B
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