Which of the following would not possible to address using a Northern Blot?
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Biotechnology Engineering · all questionsMethods and Techniques in Applied Biology
Methods and Techniques in Applied Biology
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In southern blotting . . . . . . . . is separated by gel electrophoresis
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A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be
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Terminal transferase is used
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Mung bean nuclease could be used for
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Many plasmids have Ampicillin marker. This implies
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DNA synthesis can be measured by estimating incorporation of radio-labelled
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In agarose gel electrophoresis
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A cloning vector consisting of CoS site inserted in a plasmid, used to clone DNA fragments of λ phage is
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Northern blotting is developed by
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Which of the following is incorrect?
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A gene that does not contain introns has an Eco RI site just down stream of the translational termination codon in the 3' untranslated region. The size of the gene is 4 Kbp. The length of 3' UTR is 2 Kbp, the 5' UTR is 1 Kbp and that the open reading frame is 1kbp. Upon probing a southern blot of an Eco RI digested genomic DNA with the radioactively labelled fragment containing only the open reading frame, the size of the band detected by hybridization would be
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Cells deficient in hypoxanthine guanine phospho-ribosyl transferase (HGPRT)
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Which of the following statements gives a correct explanation for the use of vectors containing drug resistance genes in the cloning of recombinant DNA (cDNA) molecules?
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There are two different ways of determining the nucleotide sequence of a nucleic acid: the chemical quencing (Maxam-Gilbert) method and the enzymatic sequencing (Sanger) method. The basic principle/advantage of the Sanger method is
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The ability to clone an adult animal from the nucleus of a differentiated somatic cell proves that
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For protoplast fusion to be successful in plant cells.
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The process used for separation of protein in polyacrylamide gel is called:
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The technique which involves addition or deletions of genes is
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In Southern blotting, DNA is fixed on
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