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Biotechnology Engineering · Q15

Methods and Techniques in Applied Biology

Engineering and GATE · Biotechnology Engineering · question 15

Q15

There are two different ways of determining the nucleotide sequence of a nucleic acid: the chemical quencing (Maxam-Gilbert) method and the enzymatic sequencing (Sanger) method. The basic principle/advantage of the Sanger method is

A.
The differential interaction of the bases with particular dyes
B.
Extension of a synthetic primer and reliable termination of DNA repair synthesis
C.
The correlation of restriction sites with the end-label of the DNA
D.
The ability to 'sequence' both strands of the DNA duplex simultaneously
Answer

Answer: Option D

Solution

Answer: Option D
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