Q75
Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?
A.
Denaturing polyacrylamide gel electrophoresis
B.
Size-exclusion chromatography
C.
Ion-exchange chromatography
AnswerD.
Nickel affinity chromatography
Answer: Option C
Solution
Answer: Option C
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