Which of the following techniques is best suited for immobilizing an affinity ligand?
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Biotechnology Engineering · all questionsBioprocess Engineering and Process Biotechnology
Bioprocess Engineering and Process Biotechnology
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Which one of the following can not be a limiting substrate if Monod's growth kinetics is applicable?
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During cultivation of microorganisms in a fermenter, various parameters are controlled by appropriate sensor (probe). Match each probe in Group I with the appropriate response mechanism in Group II.
| Group I (Probe) | Group II (Response) |
| P. Thermistor | 1. Activation of acid/alkali pump |
| Q. Oxygen electrode | 2. Activation of vegetable oil pump |
| R. Metal rod | 3. Activation of hot/cold water pump |
| S. pH electrode | 4. Increase/decrease in stirrer motor speed |
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The kinetics of the disintegration of baker's yeast cells in a bead mill is described as , where P is the concentration of protein released, m is the maximum protein concentration achievable, K is the first order rate constant and is 0.5 h−1 for the system studied. The time required for the release of 90% of the intracellular proteins will be:
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A mixture contains three similarly sized peptides P, Q and R. The peptide P is positively charged, Q is weakly negative, and R is strongly negative. If this mixture is passed through an ion-exchange chromatography column containing an anionic resin, their order of elution will be
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Match the entries in Group I with the methods of sterilization
in Group II.
| Group I | Group II |
| P. Serum | 1. Autoclave |
| Q. Luria broth | 2. Membrane filtration |
| R. Polypropylene tubes | 3. UV irradiation |
| S. Biological safety cabinets | 4. Gamma irradiation |
| 5. Dry heat |
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Match the microbial growth characteristics in Group I with the corresponding features in Group II.
| Group I | Group II |
| P. Growth associated product formation | 1. Specific growth rate decreases with increasing product concentration |
| Q. Non-growth associated product formation | 2. Specific product formation rate is constant |
| R. Product inhibition | 3. Specific product formation rate is proportional to specific growth rate |
| S. Substrate inhibition | 4. Specific growth rate decreases with increasing substrate concentration |
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In a chemostat, evaluate the dilution rate at the cell washout condition by applying Monod's model with the given set of data:
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Lysine is being produced in a lab-scale reactor by a threonine auxotroph. After 2 weeks of operation it was observed that the concentration of lysine in the reactor was gradually decreasing. Microbiological assays of reactor samples showed absence of contamination and recorded data showed no change in the operating conditions. The most probable reason for decrease in lysine concentration may be attributed to
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In a fed-batch culture, 200 g L-1 glucose solution is added at a flow rate of 50 L h-1. The initial culture volume (at quasi steady state) and the initial cell concentration are 600 L and 20 g L-1, respectively. The yield coefficient is 0.5 g cell mass-g substrate-1. The cell concentration (g L-1) at quasi steady state at t = 8 h is
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The following crosslinking agents may be used for the immobilization of enzymes.
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Thermal death of microorganisms in the liquid medium follows first order kinetics. If the initial cell concentration in the fermentation medium is 108 cells mL-1 and the final acceptable contamination level is 10-3 cells, for how long should 1 m3 medium be treated at temperature of 120°C (thermal deactivation rate constant = 0.23 min-1) to achieve acceptable load?
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Match separation methods in Group I with associated properties in Group II.
| Group I | Group II |
| P. Centrifugation | 1. Density |
| Q. Dialysis | 2. Diffusivity |
| R. Solvent extraction | 3. Size |
| S. Ultrafiltration | 4. Solubility |
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In dead-end filtration, rate of filtration is
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Which one of the following statements is correct about proportional controllers?
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A batch culture fermentation was being conducted with Streptomyces rimosus, Analysis of samples collected indicated doubling of cell number per unit time. Your interference would be that the culture is in the
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In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is 20 g L-1 (DCW), the specific growth rate (μ) is 0.4 h-1 and substrate uptake rate (v) is 16 g L-1 h-1. The cell yield coefficient will be
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The separation principle of dialysis used in the recovery of fermentation products is
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Evaluate the Michaelis constant for the following lipase catalyzed transesterification reaction for the production of biodiesel.
where k1 = 3 × 108 M−1 s−1; k−1 = 4 × 104 s−1 and k2 = 2 × 103 s−1
where k1 = 3 × 108 M−1 s−1; k−1 = 4 × 104 s−1 and k2 = 2 × 103 s−1
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Which of the following is employed for the repeated use of enzymes in bioprocesses?
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