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Biotechnology Engineering · Q42

Methods and Techniques in Applied Biology

Engineering and GATE · Biotechnology Engineering · question 42

Q42

Which statement correctly describes sequential steps in cDNA cloning?

A.
Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector
Answer
B.
mRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase, ligation to vector
C.
mRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector
D.
Double stranded cDNA synthesis, restriction enzyme digestion, addition of linkers, ligation to vector

Answer: Option A

Solution

Answer: Option A
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