Which one of the following techniques can be used to determine the structure of a 15 kDa globular protein at atomic resolution?
A. Raman spectroscopy
B. IR spectroscopy
C. UV spectroscopy
D. NMR spectroscopy
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Which of the following has to be computed to determine transmittance and absorbance at various frequencies?
A. Ratio of signal and noise
B. Ratio of sample and reference spectra
C. Sample spectra
D. Reference spectra
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Which of the following is the function of the chopper in Atomic Absorption Spectroscopy?
A. To split the beam into two
B. To break the steady light into a pulsating light
C. To filter unwanted components
D. To reduce the sample into atomic state
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Which of the following is not a fuel used in flame photometry?
A. Acetylene
B. Propane
C. Hydrogen
D. Camphor oil
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Determine the correctness or otherwise of the following Assertion (A) and the Reason (R) .
Assertion: MTT assay is used to determine cell viability based on the principle of color formation by DNA fragmentation.
Reason: MTT assay is used to determine cell viability based on the color development by converting tetrazolium soluble sail to insoluble salt.
A. Both (A) and (R) are true and (R) is the correct reason for (A)
B. Both (A) and (R) are true and (R) is not the correct reason for (A)
C. (A) is true but (R) is false
D. (A) is false but (R) is true
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Which of the following is not a limitation of Beer Lambert's law, which gives the relation between absorption, thickness and concentration?
A. Concentration must be lower
B. Radiation must have higher bandwidth
C. Radiation source must be monochromatic
D. Does not consider factors other than thickness and concentration that affect absorbance
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Phototubes are more sensitive than photovoltaic cells.
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Velocity of electromagnetic radiation is more in a vacuum than in any medium.
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Which of the following is the wavelength of microwave radiation?
A. 10 - 780nm
B. 0.78 - 30µm
C. 0.6 - 10 m
D. 0.75 - 3.75 mm
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Which of the following is not a detector used in Flame emission photometers?
A. Photronic cell
B. Photovoltaic cell
C. Photoemissive tube
D. Chromatogram
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Which of the following is not a component of the emission system in Flame photometer?
A. Burner
B. Atomiser
C. Fuel gases and their regulation
D. Chopper
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Which of the following is the advantage of prism monochromators?
A. Dispersion is non-overlapping
B. Dispersion occurs in non-linear manner
C. Dispersion is overlapping
D. Dispersion occurs in a linear manner
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Electromagnetic radiation can travel through a vacuum.
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Which of the following is not an advantage of Laminar flow burner used in Flame photometry?
A. Noiseless
B. Stable flame for analysis
C. Efficient atomization of sample
D. Sample containing two or more solvents can be burned efficiently
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The representation of Beer Lambert's law is given as A = abc. If 'b' represents distance, 'c' represents concentration and 'A' represents absorption, what does 'a' represent?
A. Intensity
B. Transmittance
C. Absorptivity
D. Admittance
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Which type of Quantum Transition takes place in Ultra Violet and Visible spectroscopy?
A. Rotation of molecules
B. Nuclear
C. Bonding electrons
D. Spin of nuclei in a magnetic field
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Which of the following is the function of the atomiser in the emission system of Atomic Absorption Spectroscopy?
A. To split the beam into two
B. To break the steady light into pulsating light
C. To break large mass of liquid into small drops
D. To reduce the sample into atomic state
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Which of the following is a source used in spectroscopy?
A. LASER
B. Tube light
C. Sodium vapour lamp
D. Tungsten lamp
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In which of the following ways, absorption is related to transmittance?
A. Absorption is the logarithm of transmittance
B. Absorption is the reciprocal of transmittance
C. Absorption is the negative logarithm of transmittance
D. Absorption is a multiple of transmittance
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Which of the following is not a reason for laser not being generally used as a source of radiation for UV, Visible Spectroscopy?
A. High cost
B. Limited range of wavelength
C. Less intensity
D. Complex to work with
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