What step of DNA sequencing is skipped during shot-gun sequencing?
A. Computer analysis
B. Cloning of DNA fragment
C. Primer reactions
D. Mapping step
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Reporter gene is turned on when
A. Promoter gene is turned on
B. Promoter gene is turned off
C. Before promoter gene is turned on
D. Any time
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Which of the following method makes use of information that can be expressed through intra and intermolecular distances
A. Distance geography (DG)
B. Distance geometry (DG)
C. Distal geometry (DG)
D. None of the above
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The goal of . . . . . . . . is to determine the location of specific genes within the genome.
A. Cloning
B. Annotation
C. Proteomics
D. Mapping
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Today; computer program such as . . . . . . . . are used daily to search sequences form more than 260,000 organism containing over . . . . . . . . billion nucleotides
A. Mast, 190
B. BLAST, 190
C. FAST, 190
D. HARSH, 190
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Which of the following is used to read light signal
A. Photomultiplier tubes & photodiodes
B. Microchannel plates or charge-coupled devices
C. Both A and B
D. None of the above
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Which among the following is likely to be important for structure based drug design?
A. Docking simulations
B. 2D-QSAR
C. Text mining
D. Gene finding
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Bioreporters refer to
A. Reporters working on biology
B. Reporters who know biology
C. Intact, living microbial cells-genetically engineered
D. None of the above
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Which type of genomics studies the physical nature of genomes?
A. Comparative genomics
B. Structural genomics
C. Functional genomics
D. None of these
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Which among the following is a compiled programming language?
A. PERL
B. FORTRAN
C. Bash script
D. AWK
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Luciferases can be found in bacteria, algae, fungi, jellyfish, insects, shrimp and squid and the resulting light that these organisms produce is termed
A. Luminescence
B. Bioluminescence
C. Fluorescence
D. Electrophoresis
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Match the following and select the correct answer:
Column-I
Column-II
a. UPGMA
1. Protein sequence database
b. CLUSTAL
2. Phylogenetic analysi
c. SWISS-PROT
3. 3-D structure visualization
d. Ras Mol
4. Multiple sequence alignment
A. a-2, b-4, c-1, d-3
B. a-1, b-4, c-2, d-3
C. a-4, b-1, c-2, d-3
D. a-3, b-2, c-4, d-1
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The genome-wide comparative mRNA expression profile between normal and cancer cells can be studied using the microarray technology. The basic approach of this technology is
A. Hybridization of fluorescently labelled genomic DNA from human cells to normal and cancer cell cDNA immobilized onto two glass slides
B. Hybridization of fluorescently labelled cDNAs from normaland cancer cells to a single glass slide containing immobilized clones of human genomic DNA
C. Hybridization of fluorescently labelled cDNAs from normal and cancer cells to a single glass slide containing immobilized clones of human cDNAs
D. Hybridization of fluorescently labelled genomic DNA from normal and cancer cells to immobilized human cDNAs on a single glass slide
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Sequence analysis of a human gene NFI showed that a region of the protein has homology to a yeast protein called Ira. Which of the following you think is most appropriate explanation for this sequence homology?
A. The homology is of no significance
B. Human and yeast are closely related
C. The similarity might reflect a common function
D. More information is required for making any conclusion
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Which of the following is the most preferred website for searching original research articles in medicine?
A. Entrez
B. Coogle
C. MSN
D. EMBL
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Which bacterial genome was the first to be completely sequenced?
A. E. coli
B. Streptococcus pneumoniae
C. Haemophilus influenzae
D. None of the above
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Which of the following gene is most commonly found to have mutated when a cancer is detected and biosensors are used in this
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Phylogeny describes a species
A. Morphological similarities with other species
B. Reproductive compatibilities with other species
C. Evolutionary history
D. Geographic distributions
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Firefly luciferase catalyzes a reaction that produces visible light in the
A. 530 - 56 nm range
B. 550 - 575 nm range
C. 680 - 740 nm range
D. 320 - 400 nm range
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An example homology and similarity tool
A. Prospect
B. Emboss
C. Rasmol
D. BLAST
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