Biotechnology Engineering · all questions

Enzyme Engineering in Biotechnology
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Q1

Which of the following is the best method for isolating enzymes from cell free extract?

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Q2

Absolute specificity, group specificity and optical specificity are observed by chemical catalyst.

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Q3

Which of the following enzyme is used to join two DNA molecule

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Q4

The rate of enzyme catalyzed reaction decreases with an increase in ionic strength if the charges are identical.

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Q5

. . . . . . . . is an example of anionic detergent.

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Q6

Which among them is not attribute of enzymes:

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Q7

The rate equation for . . . . . . . . inhibition is given by

V = \frac{V _{m a x} [ S ]}{K _{m} ( 1 + \frac{[ P ]}{K _{p}} ) + [ S ]} .

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Q8

The equation

t_{\frac{1}{2}} = \frac{0.693}{k _{d}}

represents . . . . . . . .

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Q9

Koshland proposed which model:

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Q10

If enzyme code EC 2.6.1.1 belongs to aspartate amino transferase, then the code EC 4.3.1.3 belongs to which enzyme?

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Q11

. . . . . . . . technique is nothing but a cold or psychrophilic shock.

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Q12

Selective degradation of single stranded DNA is carried out by the enzyme

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Q13

Which of the following belongs to the class of isomerases?

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Q14

The enzyme that adds mononucleotide triphosphates to the 3' OH group of a DNA fragment is

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Q15

The RNA strand in the RNA-DNA hybrid is removed by

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Q16

. . . . . . . . is responsible for maintaining biologically native structure of an enzyme and even nucleic acid.

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Q17

. . . . . . . . mediates the synthetic and degradative reactions carried out by living organism.

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Q18

Which one among them is the example of competitive inhibition of an enzyme:

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Q19

Major drawback of utilizing enzymes is . . . . . . . .

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Q20

. . . . . . . . is a method which is highly used for lab scale procedures than large scale work.

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