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Biotechnology Engineering · all questions

Microbiology in Biotechnology
practice.

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Which one of the following is correct about microbial growth medium?

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Which of the following statements are correct?
P. Viruses can play a role in causing human cancer.
Q. A tumor suppressor gene can be turned off without any change in its DNA sequence.
R. Alteration in miRNA expression levels contributes to the development of cancer.

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Determine the correctness or otherwise of the following Assertion (A) and Reason (R).
Assertion: Gram negative bacteria show staining with safranin.
Reason: Gram negative bacteria have an outer membrane with lipopolysaccharides.

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Match the organisms in Group I with the characteristics in Group II.
Group I Group II
P. Methanococcus 1. Halophile
Q. Dunaliella 2. Acidophile
R. Sulfolobus 3. Mesophile
S. Escherichia 4. Barophile

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Match the microorganisms in Group I with their fermentation products in Group II.
Group I Group II
P. Leuconostoc mesenteroides 1. Cobalamin
Q. Rhizopus oryzae 2. Sorbose
R. Gluconobacter suboxydans 3. Dextran
S. Streptomyces olivaceus 4. Lactic acid
5. Butanol

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Diphtheria toxin, tetracycline and streptomycin inhibit

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Under stress conditions bacteria accumulate

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Match the compounds in Group I with the correct entries in Group II.
Group I Group II
P. Cyanide 1. K+ ionophore
Q. Antimycin A 2. Electron transfer from cytochrome b to cytochrome c1
R. Valinomycin 3. F1 subunit of ATP synthase
S. Aurovertin 4. Cytochrome oxidase
5. Adenine nucleotide translocase

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Which combination of the following statements is correct for cyanobacteria?
P. They can perform oxygenic photosynthesis.
Q. Usually filamentous forms are involved in nitrogen fixation.
R. Nitrogen fixation occurs in heterocyst.
S. They cannot grow in a mineral medium exposed to light and air.

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A culture of bacteria is infected with bacteriophage at a multiplicity of 0.3. The probability of a single cell infected with 3 phages is

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The most plausible explanation for a sudden increase of the respiratory quotient (RQ) of a microbial culture is that

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Match the following antibiotics in Group I with their mode of action in Group II.
Group I Group II
P. Chloramphenicol 1. Binds to DNA gyrase
Q. Norfloxacin 2. Binds to RNA Polymerase
R. Puromycin 3. Inhibits peptidyl transferase
S. Rifampicin 4. Mimics aminoacyl-tRNA

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The typical coenzyme present in the methanogens is

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Match the antibiotics in Group I with the targets in Group II.
Group I Group II
P. Sulfonamide 1. Peptidoglycan synthesis
Q. Quinolones 2. Peptide chain elongation
R. Erythromycin 3. Folic acid biosynthesis
S. Cephalosporin 4. Topoisomerase

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A microorganism isolated from a salt-rich (salt concentration ∼2 M) lake was found to possess diglycerol tetraethers, with polyisoprenoid alcohol side chains, as the major lipid component of its cell membrane. The isolated organism is

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Bacteria with two or more flagella at one or both ends are called

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Which one of the following is not a product of denitrification in Pseudomonas?

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Which of the following are true for Treponema pallidum?
P. It is the causative agent of syphilis
Q. It is a spirochete
R. It is a non-motile bacterium
S. It is generally susceptible to penicillin
Choose the correct combination.

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Match the organisms in Group I with the entries in Group II.
Group I Group II
P. Clostridium 1. Rods with teichoic acid in the cell wall
Q. Escherichia 2. Rods with endospores
R. Vibrio 3. Helical rods with flagella
S. Bacillus 4. Rods with LPS in the outer membrane
5. Curved rods with polar flagella

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Which family of viruses has single stranded DNA?

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