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Biotechnology Engineering · all questions

Basic Principles of Biotechnology
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Which of the following is a prime example of a ribonucleoprotein enzyme, the catalytic activity of which (under non-physiological conditions), resides solely in its RNA component

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Main functions of baffles in a bioreactor are
P. To prevent a vortex.
Q. To increase aeration.
R. To reduce interfacial area of oxygen transfer.
S. To reduce aeration rate.

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All the following may be methods for the inhibition of microbial growth by antibiotics except

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A feature common to almost all transmembrane proteins is

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The distribution of transmembrane protein in the palne of a cell membrane can best be visualized by which of the following:

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Broker, Chow, Roberts and P. Sharp hybridized mRNA from adenovirus to denatured adenovirus DNA and examined the resultant duplex molecules by electron microscopy. Which of the following did they observe?

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If a mutation occurred in the phosphorylation site in the CTD domain of RNA polymerase II, which of the following outcomes would be observed?

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A microorganism when viewed under a compound microscope with objective of 40X and eye piece of 10X magnification measured 400μ in length. The same microorganism when viewed under a dissecting microscope with 10X lens, would measure

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Which of the following technique is used to inactivate a gene by altering the DNA encoding it?

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Gene conversion is observed

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The physiological mechanism responsible for flowering in response to temperature

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Which of the following amino acids have side chains that are negatively charged under physiological conditions (i.e., near pH 7)?

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Histones have very high percentage of basic amino acids (15 - 30%). For this class of proteins which of the following reagents would be a suitable choice for generating peptides in determination of amino acid sequence of the protein

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Number of nucleotide present in FMN is

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If a reaction is at equilibrium, the free energy, ΔG change is

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Order the following events observed during muscle contraction in the correct sequence
1. The sarcomere shortens
2. ATP is hydrolyzed
3. Ca2+ binds to troponin
4. ATP binds to myosin
5. Mosin contracts F-actin
6. F-actin moves
7. Tropomyosin moves
8. Ca2+ is released from the sarcoplasmic reticulum

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Under standard temperature (T) and pressure (P) conditions, 128 g of an ideal gas molecule A occupies a volume of 1 L. The gas molecule A obeys the relationship RT = 0.25 PV. R and V are universal gas constant and ideal gas volume, respectively. The molecule A is

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A peptide has the sequence Glu-His-Trp-Ser- Gly-Leu-Arg-pro-Gly. What is the net charge of the molecule at PH 11.

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Which of the following statements are correct? Very long DNA fragments (> 100 kb) from eukaryotic genomes
P. Can be efficiency packaged in a λ phage vector
Q. Can be propagated in yeast artificial chromosomes
R. Must have cohesive ends for cloning
S. Can be separated by pulsed field gel electrophoresis techniques

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Which one of the following statements about eukaryotic RNA polymerases I, II and III is false?

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