Henderson's equation can be respresented as
A. p H = p K a + l o g [ S a l t ] [ A c i d ]
B. p H = p K a + l o g [ A c i d ] [ S a l t ]
C. p K a = p H + l o g [ A c i d ] [ S a l t ]
D. p K a = p H − l o g [ A c i d ] [ S a l t ]
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The role of calcium in muscle contraction is to
A. Break the cross-bridges as a cofactor in the hydrolysis of ATP
B. Bind with troponin, changing its shape so that the actin filament is exposed
C. Transmit the action potential across the neuromuscular junction
D. Spread the action potential through the T tubules
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Why is the inactivation of a useful technique to determine the function of a gene?
A. Gene inactivation provides information about the expression of a gene
B. Gene inactivation provides information about the cellular location of a gene product
C. Gene inactivation provides an opportunity to identify phenotypic changes associated with the loss of the functional gene
D. Gene inactivation provides information about the structure of the gene product
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Which of the following statements is not true of fatty acids found in most mammalian cells?
A. They usually contain 12 to 20 carbons
B. Polyunsaturated fatty acids contain conjugated double bonds
C. All double bonds are in the cis configuration
D. Free fatty acids are ionized at physiological pH
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The isoelectric point of a protein is defined as
A. The pH at which the net charge on the molecule is zero
B. The pH at which all groups are protonated
C. The pH at which all groups are unprotonated
D. The pH at which each acidic group is protonated and each basic group is unprotonated
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Prandtl number is the ratio of
A. Thermal diffusivity to momentum diffusivity
B. Mass diffusivity to momentum diffusivity
C. Momentum diffusivity to thermal diffusivity
D. Thermal diffusivity to mass diffusivity
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Energy capture efficiency of the aerobic cells using glucose as a substrate is
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For a nucleic acid probe to hybridize, which of the following must be true?
A. Both the probe and the sequence to which it hybridizes must be RNA
B. Both the probe and the sequence to which it hybridizes must be DNA
C. The sequence to which it hybridizes must be identical to that of the probe
D. The sequence to which it hybridizes must be complementary to that of the probe
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A homogeneous protein of native molecular weight 100,000 gave a single band of molecular weight 50,000 on SDS-PAGE in presence of β-mercaptoethanol. N-terminal analysis gave two amino acids alanine and leucine in equal proportions. Hence the
A. Protein is a homodimer
B. Protein is contaminated with another protein
C. Protein has two polypeptides linked by disulphide bridges
D. None of the above
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In the RNAi regulatory pathway, the DICER enzyme cleaves
A. RNA polymerase into non-functional pieces
B. Single-stranded DNA into repetitive sequence
C. Double-stranded RNA into short strands
D. mRNAs of genes to be repressed
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Which one of the following is the unit of heat transfer coefficient?
A. W m2 K-1
B. W m-2 K
C. W m-2 K-1
D. W m2 K
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Amplified fragment length polymorphism (AFLP)
P. A PCR-based tool
Q. Uses restriction enzymes to cut genomic DNA
R. Involves ligation of adaptors to the sticky ends of the restriction fragments
S. Involves amplification using primers complementary to the adaptor
A. P and Q
B. R and S
C. P, Q and S
D. All of these
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Which of the following statements are true in case of fluid-mosaic model for cell membrances
P. Between 5 - 8 nm thick and appear trilaminar when viewed in cross-section under electron microscope
Q. Less than 1 nm thick and consist of a layer of protein sandwitched between two layers of phospholipids
R. In the lipid bilayer, proteins are embedded at irregular intervals and held by hydrophilic interactions between lipids and hydrophilic domains of the proteins
S. The protein domains exposed on one side of the lipid bilayer are different from those exposed on the other side
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Which one of the following is the most suitable type of impeller for mixing high viscosity (viscosity > 105 cP) fluids?
A. Propeller
B. Helical ribbon
C. Paddle
D. Flat blade turbine
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Which of the following statements about myosin is not true?
A. Myosin binds to polymerized actin
B. In vitro, myosin assembles spontaneously into the thin filaments
C. Myosin is an ATPase
D. Myosin has domains that interact with one another to effect its physiological functions
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Which of the following statements about histones is incorrect?
A. They are highly basic because they contain many positively charged amino acid side chains
B. They are extensively modified after their translation
C. In combination with DNA, they are the primary constituents of chromatin
D. They account for approximately one-fifth of the mass of a chromosome
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Microsatellites are used more commonly than minisatellite as DNA markers because
A. Minisatellites are present in too many locations within the genomes
B. Restriction enzymes can be used to type microsatellites but not minisatellite
C. There are very few microsatellites in eukaryotic genomes so they are easily identified and analyzed
D. Microsatellites are present throughout eukaryotic genomes and are easily amplified using PCR
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Which of the following Chemotherapeutic agents works by impairing de novo synthesis?
A. Acyclovir (acycloguanosine)
B. 5-fluorouracil (antimetabolite)
C. Methotrexate (antifolate)
D. Hydroxyurea
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A multiple cloning site
A. Contains many copies of a cloned gene
B. Allows flexibility in the choice of restriction enzymes for cloning
C. Allows flexibility in the choice of organism for cloning
D. Contains many copies of the same restriction enzyme site
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X-ray crystallography are
P. Based on the elastic scattering of X-rays
Q. Non-destructive analytical techniques
R. Used in determining the arrangement of atoms within a crystal
S. Based on Bragg's law
A. P and Q
B. R and S
C. P, Q and S
D. All of the above
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