Bioprocess Engineering and Process Biotechnology MCQs with Answers 2027

Q41

Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, ρ, of a double stranded Cs⁺ DNA is given by the equation ρ = 1.66 + 0.098 X_{G + C}, where X_{G + C} denotes

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Q42

Open question

Match the instruments in Group I with their corresponding measurements in Group II.

Group I	Group II
P. Manometer	1. Agitator speed
Q. Rotameter	2. Pressure difference
R. Tachometer	3. Cell number
S. Hemocytometer	4. Air flow rate

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Q43

Open question

Autoclaves are routinely used in laboratories for sterilization. They act by

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Q44

Open question

Determine the correctness or otherwise of the following Assertion (A) and the Reason (R).
Assertion: In synchronous culture, majority of the cells move to next phase of the cell cycle simultaneously.
Reason: Synchronous culture could be obtained by starving cells for essential nutrient components.

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Q45

Open question

In balanced growth phase of a cell,
P. All components of a cell grow at the same rate.
Q. Specific growth determined by cell number or cell mass would be the same.
R. The growth rate is independent of substrate concentration.
S. The growth rate decreases with decreasing substrate concentration.

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Q46

Open question

Which of the following factors can influence the lag phase of a microbial culture in a batch fermenter?
P. Inoculum size
Q. Inoculum age
R. Medium composition

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Q47

Open question

Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following (given, N = rotations/time, D = diameter of impeller)

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Q48

Open question

Match the properties in Group I with the downstream operations in Group II.

Group I	Group II
P. Size	1. Extraction
Q. Density	2. Distillation
R. Volatility	3. Filtration
S. Solubility	4. Sedimentation

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Q49

Open question

The diameters of a large and a small vessel are 1.62 m and 16.2 cm, respectively. The vessels are geometrically similar and operated under similar volumetric agitated power input. The mixing time in the small vessel was found to be 15 s. Determine the mixing time (in seconds) in the large vessel.

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Q50

Open question

Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation?

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Q51

Open question

Which of the following statements are true about bioreactors?
P. Continuous bioreactors provide less degree of control and uniform product quality than batch bioreactors.
Q. Batch bioreactors are ideally suited for reaction with substrate inhibition.
R. Choice of a bioreactor is dictated by kinetic considerations.
S. Fed batch bioreactors are also called semi-batch bioreactors.

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Q52

Open question

Which of the following statements is always correct about an ideal chemostat?
P. Substrate concentration inside the chemostat is equal to that in the exit stream.
Q. Optimal dilution rate is lower than critical dilution rate.
R. Biomass concentration increases with increase in dilution rate.
S. Cell recirculation facilitates operation beyond critical dilution rate.

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Q53

Open question

Which of the following statements applies to the operation of a fed-batch process?

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Q54

Open question

Wash out (as defined by D = μ_max) of a continuous stirred tank fermenter is characterized by (X = biomass, S = substrate concentration in bioreactor, S₀ = substrate concentration in feed, P = product concentration in bioreactor).

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Q55

Open question

Match each parameter in Group I with the appropriate measuring device in Group II.

Group I	Group II
P. Pressure	1. Photometer
Q. Foam	2. Rotameter
R. Turbidity	3. Diaphragm gauge
S. Flow rate	4. Rubber sheathed electrode

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Q56

Open question

To optimize the bioreactor system, which one of the following conditions is least important for anaerobic fermentation?

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Q57

Open question

Determine the correctness or otherwise of the following Assertion (A) and Reason (R).
Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation.
Reason: Immobilization protects the plant cells from shear forces in the bioreactor.

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Q58

Open question

Which of the following statements is incorrect about immobilized plant cell cultures?

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Q59

Open question

Which one of the following relations holds true for the specific growth rate (μ) of a microorganism in the death phase?

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Q60

Open question

Match the coefficients in Group I with their corresponding downstream processing Steps given in Group II.

Group I	Group II
P. Sedimentation coefficient	1. Aqueous two-phase extraction
Q. Partition coefficient	2. Ultrafiltration
R. Rejection coefficient	3. Dialysis
S. Activity coefficient	4. Centrifugation

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Bioprocess Engineering and Process Biotechnologypractice.

Bioprocess Engineering and Process Biotechnology
practice.