Biotechnology Engineering · all questions

Bioprocess Engineering and Process Biotechnology
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Q81

An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than one could be apparently due to one of the following reasons. Identify the correct reason.

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Q82

If the dissociation constant for solute-adsorbent binding is K_D, the retention time of the solute in a chromatography column

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Q83

In large scale fermentation process, air is sterilized by

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Q84

In large-scale fermentation, the preferred method of sterilization is

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Q85

Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm^-1. At a concentration of 50 mg L^-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm^-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L^-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:

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Q86

Match the entries in Group I with the process parameters in Group II.

Group I	Group II
P. Clark electrode	1. Liquid level
Q. Redox probe	2. Dissolved oxygen concentration
R. Load cell	3. Vessel pressure
S. Diaphragm gauge	4. pH (anaerobic process)

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