Enzymes discriminate between similar parts of same molecules. This is termed as . . . . . . . .
A. Stereospecificity
B. Absolute specificity
C. Regiospecificity
D. Group specificity
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The enzyme minus its coenzyme known as:
A. Apoenzyme
B. Metalloenzyme
C. Isoenzyme
D. All of these
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Zn+2 is an inorganic activator for enzymes:
A. Carbonic anhydrase
B. Phosphatase
C. Chymotrypsin
D. Maltase
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Freezing technique is not preferred unless otherwise there is a need.
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The . . . . . . . . inhibition gives the following rate equation.
V = K m + [ S ] ( 1 + K s [ S ] ) V m a x [ S ]
A. Substrate
B. Mixed
C. Non-competitive
D. Competitive
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Which of the following is cationic detergent?
A. Sodium dodecyl sulphate (SDS)
B. Cetyl triethyl ammonium bromide
C. Sodium lauryl sulphate (SLS)
D. Triton-X-100
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Enzymes are amphoteric molecules.
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Zymogen or proenzyme is a:
A. Modulator
B. Vitamin
C. Enzyme precursor
D. Hormone
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The following rate equation represents . . . . . . . . inhibition.
V = K m ( 1 + K i I ) + [ S ] V m a x [ S ]
A. Competitive
B. Mixed
C. Non-competitive
D. Uncompetitive
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Enzymes are made Up of:
A. Fats
B. Proteins
C. Nucleic acids
D. Vitamins
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The techniques which adopts the hypertonic and hypotonic condition for cell lysis is referred to as . . . . . . . .
A. EDTA treatment
B. Ultrasonification
C. Osmotic shock
D. Homogenization
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Phytanase can be extracted only from . . . . . . . . species.
A. Plant
B. Prokaryote
C. Fungal
D. Animal
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Catalyst discovered from biological sources are referred to as . . . . . . . .
A. Vitamins
B. Carbohydrates
C. Enzymes
D. Nucleic acids
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Enzymes that catalyze the transfer of atom or group between two molecules is known as . . . . . . . .
A. Oxidoreductases
B. Transferases
C. Ligases
D. Isomerases
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In . . . . . . . . treatment, certain molecules interact through ionic or Vander wall forces which leads to solubilization of phospholipids and lipoprotein which in turn, increases membrane permeability directing to cell lysis.
A. Lysozyme
B. EDTA
C. Detergent
D. NaoH
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Which of the following is an RNA dependent DNA synthetase
A. DNA polymerase I
B. DNA polymerase II
C. Reverse transcriptase
D. All of these
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The pH at which the net charge on the enzyme molecule is zero is called . . . . . . . .
A. pKa
B. Half-life
C. Isoelectric point
D. Km
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The technique in which the cells are subjected to cold temperature of about -15° C to -25° C is referred to as . . . . . . . .
A. Ultrasonics
B. Freezing
C. Homogenization
D. Osmotic shock
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Which of the following is an advantage of enzymes over chemical catalysts?
A. High productivity, less material cost
B. High cost of enzyme isolation
C. High cost of enzyme purification
D. Unstable nature of enzymes
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What happens to Vmax and Km in case of uncompetitive inhibition when I = Ki '?
A. Lowers to 0.5 Vmax and 0.5 Km
B. Vmax is unchanged and Km increases 2Km
C. Lowers to 0.5 Vmax and Km remains unchanged
D. Lowers to 0.67 Vmax and Km increases to 2Km
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