Which of these is correct for mixed inhibition?
A. Lowers to 0.5 Vmax and 0.5 Km
B. Vmax is unchanged and Km increases 2Km
C. Lowers to 0.5 Vmax and Km remains unchanged
D. Lowers to 0.67 Vmax and Km increases to 2Km
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The enzyme which hydrolyses starch to maltose is:
A. Protease
B. Amylase
C. Lactase
D. Maltase
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Enzyme complex involved in alcoholic fermentation is:
A. Zymase
B. Invertase
C. Lipase
D. Amylase
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Which one if the chemicals is not used to isolate enzymes from cell free extract?
A. 1.4% buffered sucrose
B. Cetyl trimethyl ammonium bromide
C. Nucleases
D. Streptomycin sulphate
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The increase in pH affects the inter H2 bonds and intramolecular hydrophobic bonds are majorly affected leading to denaturation of DNA.
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What is the most important pre-requisite for a commercial enzyme production enterprise?
A. Cheap source
B. Extraction
C. Purification
D. Isolation
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Gram positive bacteria are highly susceptible to lysozyme treatment.
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. . . . . . . . is an enzyme, which is highly produced by egg white and lachrymal glands.
A. Amylases
B. Lysozyme
C. Invertase
D. Protease
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If Ka for acetic acid is 1.78*10-5 , the pKa of acetic acid is . . . . . . . .
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Protease is an intracellular enzyme.
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Trypsin exhibits which kind of specificity?
A. Absolute specificity
B. Group specificity
C. Stereospecificity
D. Regiospecificity
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Loss of activity which may be restored by the removal of inhibitor is referred to as . . . . . . . .
A. Reversible inhibition
B. Irreversible inhibition
C. Competitive inhibition
D. Mixed inhibition
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The enzyme which specifically catalyzes β (1 → 4) glycosidic linkages of NAM (N-acetyl muramic acid) and NAG (N-acetyl glucosamine) present in bacterial peptidoglycan is . . . . . . . .
A. Protease
B. Lipase
C. Lysozyme
D. Cellulase
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Which one of the following is not a factor that limit the selection of right starting material for enzyme production?
A. Source selection
B. Availability of enzyme in the source
C. Isolation procedures
D. Location of enzyme
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Cetyl trimethyl ammonium bromide is a cationic detergent.
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Homogenization performed by passing the cells at high pressure through narrow orifice which can also cause cell rupture is referred to as . . . . . . . .
A. Ultrasonicator
B. Freezer
C. Mortar and pestle
D. French press
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Klenow enzyme is the product of enzymatic breakdown of
A. DNA polymerase I
B. DNA polymerase II
C. DNA polymerase III
D. RNA polymerase
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In . . . . . . . . treatment, produced basic radicals mediate a nucleophilic attack on compounds of cell wall on membrane leading to destabilization and fragmentation of membrane followed by release of enzymes.
A. EDTA
B. Osmotic shock
C. Alkali
D. Detergent
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Optimum pH value of pepsin is:
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Substances which reduce the rate of enzyme catalyzed reactions are known as . . . . . . . .
A. Substrates
B. Enzymes
C. Products
D. Inhibitors
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