Which of the following is not a physical method for extraction of enzymes?
A. Osmotic shock
B. Ultrasonication
C. Homogenization
D. Freezing technique
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The following rate equation is given by . . . . . . . . inhibition.
V = ( 1 + K i ′ [ I ] K m ) + S ( 1 + K i ′ [ I ] V m a x ) [ S ]
A. Mixed
B. Competitive
C. Uncompetitive
D. Non-competitive
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Which enzyme hydrolysis casein?
A. Chymosin
B. Glucose oxidase
C. Alanine aminotransferase
D. Histidase
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Which chemical is used for extracting enzymes by osmotic shock treatment?
A. EDTA
B. NaOH
C. SDS
D. 1.4% buffered sucrose
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Which of the oxidoreductases are involved in oxygen transfer form molecular oxygen?
A. Peroxidases
B. Oxidases
C. Oxygenases
D. Dehydrogenases
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What is the rate of denaturation kd , if the half-life of an enzyme catalyzed reaction is 15.6 mins?
A. 4.4*10-2 /sec
B. 0.4*102 /min
C. 4.4*10-2 /min
D. 0.4*102 /sec
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Extraction of enzymes by physical methods include the usage of alkalis, EDTA, lysozyme, etc.
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The phenomenon wherein the osmotic pressure created due to presence of solutes inside and outside the cells will be equal is referred to as . . . . . . . .
A. Isotonic condition
B. Hypertonic condition
C. Hypotonic condition
D. Osmotic shock
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This statement about enzymes is true:
A. Enzymes accelerate reactions by lowering the activation energy
B. Enzymes are proteins whose three-dimensional form is key to their function
C. Enzymes do not alter the overall change in free energy for a reaction
D. All of these
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Mercury causes irreversible inhibition.
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Homogenization utilizing . . . . . . . . is one where in manual pressure is created for cell lysis.
A. Mortar and pestle
B. Ultrasonicator
C. Freezer
D. French press
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Enzyme catalysing rearrangement of atomic grouping without altering molecular weight or number of atom is:
A. Ligase
B. Isomerase
C. Oxidoreductase
D. Hydrolase
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Enzyme which can be extracted from mammary gland is . . . . . . . .
A. Pectinase
B. Glucornidase
C. Lipase
D. Acetyl CoA carboxylase
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. . . . . . . . is a phenomenon of assuming frequencies more than 20 kHz which is above the human audibility.
A. Homogenization
B. Ultrasonics
C. Alkali treatment
D. Freezing
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Increase or decrease in . . . . . . . . leads to protonation or deprotonation of bases which leads to melting of DNA in turn isolating the enzyme after centrifugation.
A. Temperature
B. Ionic strength
C. pH
D. Detergent use
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Inhibition of invertase by sucrose falls into which category of inhibition?
A. Substrate inhibition
B. Non-competitive inhibition
C. Product inhibition
D. Competitive inhibition
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Homogenization cannot be done by using which one of the following?
A. Homogenizer
B. Mortar and pestle
C. French press
D. Ultrasonicator
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