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Biotechnology Engineering · all questions

Molecular Biology and Genetics
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RNA is very much susceptible to hydrolysis in alkali because

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Rho-dependent and rho-independent transcription termination mechanisms operate in prokaryotes. Rho independent termination mechanism involves

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Determine the correctness or otherwise of the following Assertion (A) and the Reason (R).
Assertion: Cytoplasmic male sterility (cms) is invariably due to defect(s) in mitochondrial function.
Reason: cms can be overcome by pollinating a fertility restoring (Rf ) plant with pollen from a non cms plant.

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Mobile genetic elements present in human genome are
P. Long interspersed elements (LINEs)
Q. Short interspersed elements (SINEs)
R. P elements
S. IS elements

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The direction of shell coiling in the snail Limnaea peregra is a classic example of

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Which of the following characteristics with respect to bacterial DNA polymerase III are true?
P. Initiation of chain synthesis
Q. 5' → 3' polymerization
R. 3' → 5' exonuclease activity
S. 5' → 3' exonuclease activity

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The protein in eukaryotes which is subjected to degradation undergoes

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In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand by

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Which one of the following is coded by the ABO blood group locus in the human genome?

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Determine the correctness or otherwise of the following Assertion (A) and the Reason (R).
Assertion: The association constant in water for the G-C base pair is three times lower than that for the A-T base pair.
Reason: There are three hydrogen bonds in the G-C base pair and two in the A-T base pair.

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5' capping of mRNA transcripts in eukaryotes involves, the following events:
P. Addition of GMP on the 5' end.
Q. Removal of γ-phosphate of the triphosphate on first base at the 5' end.
R. 5' - 5' linkage between GMP and the first base at 5' end.
S. Addition of methyl group to N7 position of guanine.
Which one of the following is the correct sequence of events?

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Determine the correctness of the following statements.
I. Enhancer sequences are those DNA sequences that are involved in increasing the rate of DNA replication.
II. Enhancer sequences work by binding with eukaryotic gene activator factors.

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Based on their function, find the odd one out

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For their efficient translation, eubacterial mRNAs possess a Shine-Dalgarno sequence for its recognition by an anti-Shine-Dalgarno sequence (ASD) in the ribosomes. The correct statement is

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In transgenics, alterations in the sequence of nucleotide in genes are due to
P. Substitution
Q. Deletion
R. Insertion
S. Rearrangement

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Peptidyl transferase activity resides in

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A DNA sequence, 5'-ATGGACGTGCTTCCCAAAG-CATCGGGC-3', is mutated to obtain
P. 5'- ATGGACGTGCTTCaCAAAGCATCGGGC -3'
Q. 5'- ATGGACGTGCTTCCCgAAAGCATCGGGC -3'
R. 5'- ATGGACGTGCTTCC-AAAGCATCGGGC -3'
S. 5'- ATGGACGTGCTTCCCAAtGCATCGGGC -3'
T. 5'- ATGGACGaGCTTCCCAAAGCATCGGGC -3'
[Point mutations are shown in the lower case or '-' within the sequences]
Which of the above mutant sequences do not have frameshift?

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DNA helicases catalyze the reaction

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The formation of peptide cross links between adjacent glycan chains in cell wall synthesis is called

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In a relatively large but finite and closed population of sexually reproducing diploid organisms, the frequency of homozygous genotype PP changes from 0.40 to 0.50 and that of pp changes from 0.40 to 0.41 in a span of 10 generations. Which of the following is the most likely cause for the above change in frequency of the PP genotype?

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