Molecular Tools in Biotechnology MCQs with Answers 2027

Q21

For the sequence of dsDNA given below, identify the set of primers required to amplify this DNA by PCR.
3' GACTCCA . . . . . . . . TACAACC 5'
5' CTGAGGT . . . . . . . . ATGTTGG 3'

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Q22

Open question

Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).
5'-GACCTGTGG . . . . . . . . ATACGGGAT-3'
3'-CTGGACACC . . . . . . . . TATGCCCTA-5'
Primers
P. 5'-GACCTGTGG-3'
Q. 5'-CCACAGGTC-3'
R. 5'-TAGGGCATA-3'
S. 5'-ATCCCGTAT-3'

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Q23

Open question

Protein binding regions of DNA are identified by one of the following techniques.

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Q24

Open question

Nick translation of DNA is a method for making DNA probes. Identify from below what is not required for nick translation method.

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Q25

Open question

Match the reagents in Group I with their preferred cleavage sites in Group II.

Group I	Group II
P. Cyanogen bromide	1. Carboxyl side of methionine
Q. o-Iodosobenzoate	2. Amino side of methionine
R. Hydroxylamine	3. Carboxyl side of tryptophan
S. 2-Nitro-5-thiocyanobenzoate	4. Amino side of cysteine
	5. Asparagine-glycine bonds

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Q26

Open question

The mobility of DNA in agarose gel electrophoresis is solely based on its

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Q27

Open question

A polymerase chain reaction was performed beginning with 400 template DNA molecules in a 100 μL reaction. After 20 cycles of polymerase chain reaction, how many molecules of the amplified product will be present in 0.1 μL of reaction?

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Q28

Open question

A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication?

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Q29

Open question

All of the following are true about DNA microarray technology except

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Q30

Open question

Expressed Sequence Tag is defined as

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Q31

Open question

Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially?

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Q32

Open question

CRISPR-Cas system is associated with

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Q33

Open question

Identify the correct statements.
P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction.
Q. S1 nuclease can cleave the DNA strand of a DNARNA hybrid.
R. T4 polynucleotide kinase is used for labeling 3' end of DNA.
S. Baculovirus (Autographa californica) can be used as an insect expression vector.

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Q34

Open question

W, X and Y are the intermediates in a biochemical pathway as shown below:

S \cdot \cdot \cdot \cdot \cdot \cdot \cdot \cdot \to W \to X \to Y \to Z

Mutants auxotrophic for Z are found in four different complementation groups, namely Z1, Z2, Z3 and Z4. The growth of these mutants on media supplemented with W, X, Y or Z is shown below (Yes: growth observed: No: growth not observed):

Mutants	Media Supplemented with Mutants
Mutants	W	X	Y	Z
Z1	No	No	Yes	Yes
Z2	No	Yes	Yes	Yes
Z3	No	No	No	Yes
Z4	Yes	Yes	Yes	Yes

What is the order of the four complementation groups in terms of the step they block?

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Q35

Open question

For amplification of GC rich sequences by polymerase chain reaction, identify the reagent that hinds and stabilizes AT sequences and destabilizes GC regions.

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Q36

Open question

In order to identify the person who committed a crime, forensic experts will need to extract DNA from the tissue sample collected at the crime scene, and conduct one of the following procedures for DNA fingerprinting analysis

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Q37

Open question

Restriction endonucleases from two different organisms that recognize the same DNA sequence for cleavage are called

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Q38

Open question

Protein-DNA interactions in vivo can be studied by

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Q39

Open question

The DNA sequence shown below is to be amplified by PCR:
5' GCTAAGATCTGAATTTTCC . . . . . . . .
TTGGGCAATAATGTAGCGC 3'
3' CGATTCTAGACTTAAAAGG . . . . . . . .
AACCCGTTATTACATCGCG 5'
Which one of the following pair of primers can be used for this amplification?

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Q40

Open question

For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated DNA?

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Molecular Tools in Biotechnologypractice.

Molecular Tools in Biotechnology
practice.