The two principal contractile proteins found in skeletal muscle are:
A. Actin and troponin
B. Actin and myosin
C. Troponin and tropomyosin
D. Myosin and tropomyosin
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The blue colonies contained
A. Plasmid only
B. Foreign DNA only
C. Both A and B
D. None of these
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Which of the following is a false term?
A. Repetitive DNA
B. Beautiful DNA
C. Unique DNA
D. Promiscous DNA
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Which one of the following media components is used to maintain pH in mammalian cell culture?
A. CaCh
B. MgSO
C. NaCl
D. NaHCO3
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Which of the following would be used to study which genes are transcribed in a particular cell line?
A. Gene expression profiling
B. DNA variation screening
C. Microarray comparative genomic hybridization
D. Transcription-mediated amplification
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A heterologous protein for its expression in the milk of a transgenic animal should be under the control of the promoter of the gene coding for
A. β-globin
B. β-lactoglobulin
C. Preproinsulin
D. lacZ
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A multicellular organism that carries a specific genetic change in each cell bacause of an intervention at the fertilized egg stage is a
A. Transversion
B. Transition
C. Transgenic
D. Transformation
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The synthesis of glucose from lactate, glycerol, or amino acids is called
A. Glycogenolysis
B. Glycolysis
C. Lipolysis
D. Gluconeogenesis
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Cells that leave a tumor and spread through out the body forming tumors at distant sites are called
A. Sarcomas
B. Metastases
C. Carcinomas
D. Lymphomas
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The number of replicons in a typical mammalian cell is
A. 40 - 200
B. 400
C. 1000 - 2000
D. 50,000 - 100,000
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In the above problem, colonies that were white in the presence of X-gal contained
A. Plasmid DNA only
B. Foreign DNA only
C. Both A and B
D. None of these
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HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following
I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine.
II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.
A. Only I is true
B. Only II is true
C. Both I and II are true
D. I is true and II is false
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Which among the following immunological techniques is based on passive diffusion of antigen and antibody towards each other
A. Rocket immunoelectrophoresis
B. Ouchterlony immunodiffusion
C. Radioimmunoassay
D. ELISA
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Alpha and Beta amylase help in
A. Biosynthesis of lipids
B. Breakdown of lipids
C. Breakdown of starch
D. Breackdown of sucrose
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Embryonic stem cells are derived from
A. Fertilized embryo
B. Unfertilized embryo
C. Sperm
D. Kidney
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Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because
A. It is an organic solvent
B. It easily penetrates cells
C. It protects cells by preventing crystallization of water
D. It is also utilized as a nutrient
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Somatic cell gene transfer is used for
P. Transgenic animal production.
Q. Transgenic diploid cell production.
R. In vitro fertilization.
S. Classical breeding of farm animals.
A. P, R and S
B. P, Q and R
C. P and R
D. P only
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Cells deficient in hypoxanthine guanine phosphoribosyl-transferase (HPRT) enzyme rely on
A. Synthesis of purine deoxynucleotides by salvage pathway
B. Synthesis of purine deoxynucleotides by de novo pathway
C. Supply of hypoxanthine in the culture medium
D. Supply of thymidine in the culture medium
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An animal cell line was transected with DNA extracted from a tumorous tissue. Which one of the following will be diagnostic for its tumorous transformation?
A. Altered cell shape
B. Contact inhibition
C. Anchorage-independent cell division
D. Increased duration of cell cycle
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Which are the most commonly used enzymes for separating cells in a given tissue for culture?
A. Trypsin
B. Collagenase
C. Both A and B
D. None of the above
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