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Biotechnology Engineering · all questions

Animal Biotechnology
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The two principal contractile proteins found in skeletal muscle are:

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The blue colonies contained

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Which of the following is a false term?

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Which one of the following media components is used to maintain pH in mammalian cell culture?

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Which of the following would be used to study which genes are transcribed in a particular cell line?

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A heterologous protein for its expression in the milk of a transgenic animal should be under the control of the promoter of the gene coding for

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A multicellular organism that carries a specific genetic change in each cell bacause of an intervention at the fertilized egg stage is a

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The synthesis of glucose from lactate, glycerol, or amino acids is called

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Cells that leave a tumor and spread through out the body forming tumors at distant sites are called

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The number of replicons in a typical mammalian cell is

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In the above problem, colonies that were white in the presence of X-gal contained

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HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following
I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine.
II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.

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Which among the following immunological techniques is based on passive diffusion of antigen and antibody towards each other

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Alpha and Beta amylase help in

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Embryonic stem cells are derived from

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Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because

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Somatic cell gene transfer is used for
P. Transgenic animal production.
Q. Transgenic diploid cell production.
R. In vitro fertilization.
S. Classical breeding of farm animals.

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Cells deficient in hypoxanthine guanine phosphoribosyl-transferase (HPRT) enzyme rely on

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An animal cell line was transected with DNA extracted from a tumorous tissue. Which one of the following will be diagnostic for its tumorous transformation?

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Which are the most commonly used enzymes for separating cells in a given tissue for culture?

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