Bacillus subtilis under ideal conditions occupies 5% of the total volume of a fermenter. Under such conditions; the culture medium (after bacilli are filtered off) has a concentration of 1mg/ml of the secreted protein trehalae
A. (i) 106 ml, (ii) 1.57 × 108
B. (i) 108 ml, (ii) 3.16 × 1018
C. (i) 105 ml, (ii) 1.57 × 108
D. (i) 106 ml, (ii) 3.18 × 1016
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In animal cell cultures, the addition of serum to media is essential for providing
A. Amino acids for protein synthesis
B. Nucleotides for DNA synthesis
C. Growth factors
D. All of the above
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A knockout mouse is so named because it contains
A. Aggression genes
B. Multiple copies of a human gene
C. An activated human gene
D. None of the above
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In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is correct?
A. Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2
B. Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2
C. Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2
D. CO2 requirement is independent of bicarbonate concentration in the medium
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Which of these would not Be-used to introduce DNA in animal cells?
A. Liposomes
B. Electroporation
C. Microinjection
D. Ti-plasrnid
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The first patent for a transgenic organism was awarded in 1988. Which organism was patented?
A. A yeast used in industrial processes
B. A bacterium able to metabolize components of crude oil
C. A mouse that manufactures human protein in its milk
D. Life forms cannot be patented
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Carbon-dioxide when combined with the amino groups in haemoglobin molecule and forms
A. HbCO2
B. HbCO
C. Hb(CO)(-)3
D. Hb(CO3 )2
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Term hybridoma implies
A. DNA-RNA hybrid
B. Recombination of DNA molecules
C. Somatic hybridisation
D. Gametic fusion
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DNA ligase of E. coli requires which of the following cofactors?
A. Flavin-adenine dinucleotide as an electron acceptor
B. Nicotinamide-adenine dinucleotide phosphate as a phosphate donor
C. Nicotinamide-adenine dinucleotide (NAD+ ) to form an active adenyl enzyme
D. (NAD+ ) as an electron acceptor
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The syndrom es in human in which an individual's somatic cells contain only the one sex chromosome XO is called
A. Klinefelter syndrome
B. Turner syndrome
C. Downs syndrome
D. Super female
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Choose the correct sequence of steps involved in cytoplast production.
A. Digestion of cell wall → Protoplast viability → Cybrid formation → Osmotic stabilizer
B. Osmotic stabilizer → Digestion of cell wall → Protoplast viability → Cybrid formation
C. Protoplast viability → Osmotic stabilizer → Digestion of cell wall → Cybrid formation
D. Osmotic stabilizer → Digestion of cell wall → Cybrid formation → Protoplast viability
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Which one of the following statements is incorrect about hybridoma production?
A. Hybridoma cells can use hypoxanthine and thymidine
B. DNA synthesis in myeloma cells is blocked by aminopterin
C. Hybridoma cells are made to produce polyclonal antibodies
D. Polyethylene glycol is used to fuse myeloma cells to B-cells
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Antibody-producing hybridoma cells are generated by the fusion of a
A. T cell with a myeloma cell
B. B cell with a myeloma cell
C. Macrophage with a myeloma cell
D. T cell and a B cell
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Which of the following has been produced commercially from mammalian cell cultures?
A. Plasminogen activator
B. Antibacterial antibiotics
C. Insulin
D. Renin
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When the value of selection coefficient is unity (S = 1), there would be
A. No survival
B. 10% survival
C. 50% survival
D. 75% survival
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To promote attachment and spreading of anchorage-dependent animal cells the surface of the culture vessel needs to be coated with
A. Trypsin
B. Collagen
C. Pronase
D. Polyglycol
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The culture fluids of 1000 to 5000 colonies of hybridoma are screened for monoclonal antibody by
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Nitrogen fixing genes are called
A. Nif' genes
B. Plasmid genes
C. Leg genes
D. Cos genes
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Transgenic pharming involves using . . . . . . . . to produce recombinant proteins in . . . . . . . .
A. Cows; Milk
B. Phage; Bacteria
C. Bacteria; Culture media
D. Fungi; Culture media
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The genes which promote cancerous growth are called as
A. Oncogenes
B. Tumour suppressor genes
C. Both A and B
D. None of the above
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