Which of the following statement is not true for Xanthine-guanine phosphoribosyl transferase (XGPRT)?
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Biotechnology Engineering · all questionsAnimal Biotechnology
Animal Biotechnology
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Production of monoclonal antibodies by hybridoma technology requires
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Choose the correct combination of True (T) and False (F) statements about microcarriers used in animal cell culture.
P. Higher cell densities can be achieved using microcarriers.
Q. Microcarriers increase the surface area for cell growth.
R. Microcarriers are used for both anchorage- and non-anchorage-dependent cells.
S. Absence of surface charge on microcarriers enhances attachment of cells.
P. Higher cell densities can be achieved using microcarriers.
Q. Microcarriers increase the surface area for cell growth.
R. Microcarriers are used for both anchorage- and non-anchorage-dependent cells.
S. Absence of surface charge on microcarriers enhances attachment of cells.
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The bacteria known to be naturally competent for transformation of DNA is
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Which of the following DNA Pol has proofreading property in animal cells?
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When electroporation is used for introducing DNA into mammalian cell
P. A carrier for DNA is not required.
Q. The lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites.
R. The viability of the cell becomes approximately zero percent.
S. The first step involves absorption of DNA on the cell membrane.
P. A carrier for DNA is not required.
Q. The lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites.
R. The viability of the cell becomes approximately zero percent.
S. The first step involves absorption of DNA on the cell membrane.
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Positional cloning approach exploits information
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Determine the correctness or otherwise of the following Assertion [A] and the Reason [R].
Assertion [A]: Embryonic stem cells are suitable for developing knockout mice.
Reason [R]: Homologous recombination is more frequent in embryonic stem cells than that in somatic cells.
Assertion [A]: Embryonic stem cells are suitable for developing knockout mice.
Reason [R]: Homologous recombination is more frequent in embryonic stem cells than that in somatic cells.
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Gene targeting is done on an
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Detection of which hormone is the commonly used test for pregnancy in humans?
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Methotrexate (MTX) resistant cell lines developed through selection show
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Hybridoma technology is used to produce
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Match the following reporter gene with their source
| Column-I | Column-II |
| A. β-Galactosidase | 1. Firefly |
| B. Lux | 2. E. coli |
| C. TK | 3. Herpers simplex virus |
| D. Blasticidin deaminase (bed) | 4. Aspergillus terrcus |
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For the growth of T-cell, the growth factor needed would be
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A hybrid derived from the fusion of a myeloma cell (HPRT−) with an antibody secreting B-lymphocyte (HPRT+) can be selected to produce monoclonal antibody by growing in a medium containing
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A bacterial culture contains 108 cells/ml in the beginning. Two hours later, it was found to contain 1012 cells/ml. Calculate the doubling time.
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r-HuEPO is
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Receptor R is over expressed in CHO cells and analyzed for expression. 6 × 107 cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm. the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor)
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Which one of the following is NOT used for the measurement of cell viability in animal cell culture?
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Gene therapy that is hereditary relates to
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