Which of the following statement is incorrect?
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Biotechnology Engineering · all questionsMethods and Techniques in Applied Biology
Methods and Techniques in Applied Biology
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RF =
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The sensitivity of ELISA is
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Hybridoma technology is used in
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Which statement about restriction enzymes are false?
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Free amino acids can be determined with the help of
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Reverse genetics means
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If the linear double stranded genomic DNA of bacteriophage T4 is heated and cooled, one gets both linear and circular molecules because,
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The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?
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The genome sequencing of rice is important because
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Match List-I (cloning vector) with List-II (character) and select the correct answer:
| List-I: Cloning vector | List-II: Character |
| a. Yeast episomal plasmid (YEp) | 1. Integrates with host chromosome for replication |
| b. Yeast integrative plasmid (YIp) | 2. Carries a chromosomal origin of replication |
| c. Yeast replicative plasmid (YRp) | 3. Carries origin of replication of the 2 mm plasmid |
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Which of the following methods would give you the most precise and accurate information about where and when a given gene is expressed?
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Which of the following are not valid methods of labelling duplex DNA?
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BAC, which can be used to clone large DNA fragments, is derived from
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Restriction endonucleases hydrolyzes polynucleotide from
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ELISA is used to detect viruses where the key reagent is
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Genetically engineered male sterile crop plants may be produced by inserting
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Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately
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Which of the following is a desirable characteristic for a cloning plasmid?
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Which of the following could not possibly give rise to a restriction fragment length polymorphism (RFLP)?
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