Restriction endonucleases which recognize and cut same recognition sequences are known as
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Biotechnology Engineering · all questionsRecombinant DNA Technology
Recombinant DNA Technology
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The length of each boarder sequence in Ti plasmid is about
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A DNA fragment of 4500 bp has to be tailed with dT residues by using dTTP and the enzyme 'terminal transferase' The stock solution of dTTP that is used as a substrate has concentration of 150 μM. Ten μL of this stock solution is added to a total volume of 200 μL reaction. What will be the concentration of dTTP in the reaction?
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Baculovirus expression system is used to express heterologous genes in
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A variety of genetic elements are used in the transgenic plant research. Match the genetic elements (Group I) with their corresponding source (Group II).
| Group I | Group II |
| P. Ubiquitin1 promoter | 1. Agrobacterium tumefaciens |
| Q. Nos transcriptional terminator | 2. Streptomyces hygroscopicus |
| R. bar selection marker gene | 3. Escherichia coli |
| S. gus reporter gene | 4. Zea mays |
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The essential component of Ti plasmid required for integration into plant genome is
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The bacteria known to be naturally competent for transformation of DNA is
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BAC, which can be used to clone large DNA fragments, is derived from
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Transfer of T-DNA from Ti plasmid into plant cell is mediated by
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Antibiotic resistance marker that cannot be used in a cloning vector in Gram negative bacteria is
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A gene cannot be isolated from a human genomic DNA library by functional complementation in E. coli because of
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During a positive-negative selection process, transformed animal cells expressing . . . . . . . . are killed in presence of ganciclovir in the medium.
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To isolate a gene coding for glucagon, the cDNA library as to be constructed using mRNA isolated from
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Which one of the following cannot be a recognition sequence for a type II restriction enzyme?
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To be a cloning vector, a plasmid does not require
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Select the correct combination of genetic components that are essential for the transfer of T-DNA segment from Agrobacterium tumefaciens to plant cells.
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A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled?
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A cell has five molecules of a rare mRNA. Each cell contains 4 × 105 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?
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The enzymes that can be used in 5' end labeling of DNA are
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The restriction endonuclease HaeIII recognizes the sequence GG↓CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice
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