DNA synthesized from an RNA template is called
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Biotechnology Engineering · all questionsRecombinant DNA Technology
Recombinant DNA Technology
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Which one of the following is not a protease?
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The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is 6 bases long can be estimated to be
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The substrate for restriction enzyme is
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Hairy roots' induced in vitro by the infection of Agrobacterium rhizogenes, are characterized by
P. A high degree of lateral branching.
Q. Genetic instability of culture.
R. An absence of geotropism.
S. Poor biomass production.
P. A high degree of lateral branching.
Q. Genetic instability of culture.
R. An absence of geotropism.
S. Poor biomass production.
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Match the items in Group I with their functions in Group II.
| Group I | Group II |
| P. rol genes | 1. Food and energy source |
| Q. Opines | 2. Tumor formation |
| R. Virulence genes | 3. Hairy root induction |
| S. aux and cyt genes | 4. T-DNA transfer and integration |
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A vector derived from which one of the following viruses is used for high-frequency genomic integration of a transgene in animal cells?
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In nature, Agrobacterium tumefaciens mediated infection of plant cells leads to
P. Crown gall disease in plants.
Q. Hairy root disease in plants.
R. Transfer of T-DNA into the plant chromosome.
S. Transfer of Ri-plasmid into the plant cell.
P. Crown gall disease in plants.
Q. Hairy root disease in plants.
R. Transfer of T-DNA into the plant chromosome.
S. Transfer of Ri-plasmid into the plant cell.
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Mung bean nuclease could be used for
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Human genome sequencing project involved the construction of genomic library in
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Which of the following processes require energy?
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A plasmid cloning vector should contain all of the following sequences except
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"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
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Consider the following statements.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.
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You have cut the genome of a double-stranded viral genome with a restriction endonuclease and electrophoresed the products on an agarose gel. You observe only one band on the gel, equivalent to the size of the genome. This is because
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Which one of the following features is not required in a prokaryotic expression vector?
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Yeast artificial chromosomes (YAC's) are used for cloning
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In baculovirus expression vector, foreign genes are expressed from the promoter of
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